It is the first time I prepare a manuscript. PI assigned to me the introduction and discussion parts. I went through the instructions in the journal we will submit. Unfortunately, I stucked on some points. The font size and bolding; title, heading, text? it is only written the preferred fonts but nothing about the size. Do I have to format the texts to be like side to side columns (like we see in newspapers and published articles) or just normal centered text, and the journal will edit that in the way they like? What about separating lines? do I have to do them like those in published articles? (e.g, I see a separating line immediately after key words, before introduction). What about references? It is only written to be like the Medline preferred styles?! Does this mean Vancouver or another style? In the end, may this question summarizes all my queries if answer is yes, Is the manuscript in preparation phase identical to the published work in the journal? or there are differences? Sorry for my silly questions!