If you are taking high resolution, multi-color digital images, you can probably see a difference, as the color corrections for chromatic aberration might not quite be the same. When doing three color fluorescent imaging, and combining three monochrome pics into a false color single image (for research purposes), I could see minor differences based on the objective. Using your eyes under usual light microscopy conditions (H & E, or immunostaining), it would be challenging to distinguish between the two, IMO.