Quick Q about electroblotting!

[Rolling_with_the_punches]

Does anyone know why a heavier protein would transfer during electroblotting but a smaller protein wouldn't?
Shouldn't smaller proteins electroblot more easily?

thanks!

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[Goro]

Depends upon how small the smaller protein was....maybe it ran off the gel?

The things >100kDa tend to be the ones that have more trouble blotting.

Does anyone know why a heavier protein would transfer during electroblotting but a smaller protein wouldn't?
Shouldn't smaller proteins electroblot more easily?

thanks!

 

[hoihaie]

what kind of gel ae you using? gradient?

 

[911 Turbo]

How do you know your protein didn't transfer? How much of the protein are you expecting in the sample?

 

[Ismet]

SDN is not for homework help. Closing thread.