Sorry to keep blowing you guys up with questions but I have another answer that I don't entirely agree with from Kaplan.
Restriction endonuclease are used for which of the following?
I. Gene Therapy
II. Southern Blotting
III. DNA Repair
A. I only B. II only C. II and III only D. I,II, and III only
So from my understanding, restriction endonucleases create double stranded breaks, like what you would need in southern blotting, and excision endonucleases make single strand breaks like what you would use for base excision and nucleotide excision repair (the two types of DNA repair). I would think that would mean the answer would be I and II which isn't a choice. Anyone have any insight into why this answer is D?