Is it more accurate to estimate Vmax from [V] vs curve, or from Lineweaver-Burk equation?
This is a question from Princeton Biochemistry Review book page 95 Chapter 4 Practice passage. In the passage, it says in second paragraph:
"Despite the advantages of this linear representation of enzymatic rate data, the Lineweaver-Burk plot introduces greater error when used to determine Vmax and Km. Thus, it is not recommended when highly accurate values of these kinetic parameters are desired.
Then question 1 is "Which of the following is the best estimate for the Vmax of the enzyme represented by Figure 1"
In figure 1, it contains both the [V] vs curve and Lineweaver-Burk representation. V vs S curve levels off between 40-45 umol/min; but y axis intersect at 0.01 (which correspond to 50 if using 1/Vmax)
The correct answer according to book is D (52 umol/min) instead of C (44 umol/min)
What I cannot understand is: the passage said specifically that L-B plot introduce greater error for determining Vmax. Why it still chooses Vmax value from L-B plot instead of V vs S plot?
This is a question from Princeton Biochemistry Review book page 95 Chapter 4 Practice passage. In the passage, it says in second paragraph:
"Despite the advantages of this linear representation of enzymatic rate data, the Lineweaver-Burk plot introduces greater error when used to determine Vmax and Km. Thus, it is not recommended when highly accurate values of these kinetic parameters are desired.
Then question 1 is "Which of the following is the best estimate for the Vmax of the enzyme represented by Figure 1"
In figure 1, it contains both the [V] vs curve and Lineweaver-Burk representation. V vs S curve levels off between 40-45 umol/min; but y axis intersect at 0.01 (which correspond to 50 if using 1/Vmax)
The correct answer according to book is D (52 umol/min) instead of C (44 umol/min)
What I cannot understand is: the passage said specifically that L-B plot introduce greater error for determining Vmax. Why it still chooses Vmax value from L-B plot instead of V vs S plot?