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So today in lab I was making a buffer. The recipe made a total volume of 1L, however, initially you add 900mL then add a few solid ingredients. After the solid ingredients are added you top off the volume to 1L and make sure the pH is 8.5.
Our lab manager claimed you should pH the solution before you top the final volume off to 1L (so after you add the solid ingredients). I didn't argue with her or anything, but that doesn't make sense to me. Adding extra water will change the pH and bring it closer to 7,no?
I'm only asking because this is bothering me, not because I'm mad about a small detail or the interaction. Anyone care to explain who was correct/why? Thanks for the replies
Our lab manager claimed you should pH the solution before you top the final volume off to 1L (so after you add the solid ingredients). I didn't argue with her or anything, but that doesn't make sense to me. Adding extra water will change the pH and bring it closer to 7,no?
I'm only asking because this is bothering me, not because I'm mad about a small detail or the interaction. Anyone care to explain who was correct/why? Thanks for the replies