1. Dismiss Notice
  2. Hey Guest! Check out the 3 MCAT Study Plan Options listed in the 'stickies' area at the top of the forums (BoomBoom, SN2ed, and MCATJelly). Let us know which you like best.

    Also, we now offer a MCAT Test-Prep Exhibitions Forum where you can ask questions directly from the test-prep services.
    Dismiss Notice
  3. This forum is for support and discussion only. Please promote test prep materials/services (including AMAs) in the Special Offers subforum only. Thanks!
    Dismiss Notice
Dismiss Notice

Interview Feedback: Visit Interview Feedback to view and submit interview information.

Interviewing Masterclass: Free masterclass on interviewing from SDN and Medical College of Georgia

EK FL1 NO. 132

Discussion in 'MCAT: Medical College Admissions Test' started by Demihorvat, Aug 24, 2015.

  1. Demihorvat

    2+ Year Member

    Oct 1, 2013
    Likes Received:
    Medical Student (Accepted)
    Hi everyone, I was wondering if anyone could help me with this question: DNA samples from an HbAA and an HbSS individual are digested with restriction endonucleases, separated by gel electrophoresis and exposed to a hybridization probe. Southern blotting shows 2 bands for the HbSS sample, but only one for the HbAA sample. Which is the most likely explanation?

    The answer is: HbSS sample contains a restriction site while the HbAA doesn't.

    My question is: why isn't it that the HbSS sample contains 2 restriction sites while HbAA contains 1?

  2. is it because 1 site equals 2 bands because it cleaves at one site creating 2 different segments, so if you had 2 sites you would have 3 bands?

Share This Page