Demihorvat

5+ Year Member
Oct 1, 2013
70
14
Status
Medical Student (Accepted)
Hi everyone, I was wondering if anyone could help me with this question: DNA samples from an HbAA and an HbSS individual are digested with restriction endonucleases, separated by gel electrophoresis and exposed to a hybridization probe. Southern blotting shows 2 bands for the HbSS sample, but only one for the HbAA sample. Which is the most likely explanation?

The answer is: HbSS sample contains a restriction site while the HbAA doesn't.

My question is: why isn't it that the HbSS sample contains 2 restriction sites while HbAA contains 1?

Thanks!
 
6

691622

is it because 1 site equals 2 bands because it cleaves at one site creating 2 different segments, so if you had 2 sites you would have 3 bands?
 
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