1. Hey Guest! Check out the 3 MCAT Study Plan Options listed in the 'stickies' area at the top of the forums (BoomBoom, SN2ed, and MCATJelly). Let us know which you like best.

    Also, we now offer a MCAT Test-Prep Exhibitions Forum where you can ask questions directly from the test-prep services.
    Dismiss Notice
  2. Download free Tapatalk for iPhone or Tapatalk for Android for your phone and follow the SDN forums with push notifications.
    Dismiss Notice
Dismiss Notice

Interview Feedback: Visit Interview Feedback to view and submit interview information.

Interviewing Masterclass: Free masterclass on interviewing from SDN and Medical College of Georgia

Dismiss Notice
Hey Texans—join us for a DFW meetup! Click here to learn more.

EK FL1 NO. 132

Discussion in 'MCAT Discussions' started by Demihorvat, Aug 24, 2015.

  1. Demihorvat

    2+ Year Member

    Oct 1, 2013
    Likes Received:
    Medical Student (Accepted)
    Hi everyone, I was wondering if anyone could help me with this question: DNA samples from an HbAA and an HbSS individual are digested with restriction endonucleases, separated by gel electrophoresis and exposed to a hybridization probe. Southern blotting shows 2 bands for the HbSS sample, but only one for the HbAA sample. Which is the most likely explanation?

    The answer is: HbSS sample contains a restriction site while the HbAA doesn't.

    My question is: why isn't it that the HbSS sample contains 2 restriction sites while HbAA contains 1?

  2. Note: SDN Members do not see this ad.

  3. is it because 1 site equals 2 bands because it cleaves at one site creating 2 different segments, so if you had 2 sites you would have 3 bands?

Share This Page