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EK FL1 NO. 132

Discussion in 'MCAT Discussions' started by Demihorvat, Aug 24, 2015.

  1. Demihorvat

    2+ Year Member

    Joined:
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    Status:
    Medical Student (Accepted)
    Hi everyone, I was wondering if anyone could help me with this question: DNA samples from an HbAA and an HbSS individual are digested with restriction endonucleases, separated by gel electrophoresis and exposed to a hybridization probe. Southern blotting shows 2 bands for the HbSS sample, but only one for the HbAA sample. Which is the most likely explanation?

    The answer is: HbSS sample contains a restriction site while the HbAA doesn't.

    My question is: why isn't it that the HbSS sample contains 2 restriction sites while HbAA contains 1?

    Thanks!
     
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  3. is it because 1 site equals 2 bands because it cleaves at one site creating 2 different segments, so if you had 2 sites you would have 3 bands?
     

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