I thought the anode in electrophoresis is negatively charged. If that's true, can someone please explain this answer to me? It's from Kaplan FL 2. An amino acid is subjected to electrophoresis at pH 8.5 and is observed to migrate to the anode. The IP point of this amino acid is: The answer is less than 8.5 because at 8.5, it has a net negative charge and is migrating toward the anode. But if amino acid is migrating toward the anode, shouldn't it have a net positive charge since the anode is negative? And if it has a net positive charge, would the IP be more than 8.5 or less? Can someone please help? I guess I don't understand isoelectric point as well as I thought I did.