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Basically looking to get a single cell suspension from whole tissues (an array of types) to use for protein and mRNA extraction...
I think in the past when I've done this, I did a collagenase digest for some time, and then extracted for proteins using a cell lysis buffer.
Anybody got any references or protocols that have worked well for them (what tissue type?)
I think in the past when I've done this, I did a collagenase digest for some time, and then extracted for proteins using a cell lysis buffer.
Anybody got any references or protocols that have worked well for them (what tissue type?)