Sorry my bad. What I meant was that the first polar body will have twice as many genetic materials as the first, if no non-disjunction took place.Under normal condition (no aneuploidy), a dye can distinguish them. I am not sure if they can be differentiated by looking at the shape of the chromosomes because that would only be possible if the chromosomes were still condensed. That may be true for the first polar body, not sure about the second. But either way, again, only possible if no aneuploidy.
Secondly, because the second polar body can only be formed AFTER the egg is fertilized, when you collect them you should know exactly which is which. What I meant earlier was that I don't see the circumstance which you can collect two types of polar body at the same time. I read around a bit and learned about polar body biopsy, which I believe is a controlled procedure
in vitro... so whoever performs it (correctly) should know exactly which polar body is under the microscope.
https://books.google.com/books?id=91R1CgAAQBAJ&pg=PA32&lpg=PA32&dq="polar+body+biopsy+1987&source=bl&ots=AQcJ35NEJC&sig=L7jhCiDKNSfSI8NC5GeVatugwQY&hl=en&sa=X&ved=0ahUKEwi-7abQusXJAhXE5YMKHXwUAW8Q6AEIHDAA#v=onepage&q="polar body biopsy 1987&f=false
If the question were to distinguish between 2 random polar bodies from different people, both of which are devoid of DNA, I don't believe it would be possible.