Net ATP from Glycolysis & TCA .... Biochemistry Accounting

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For those who have taken Biochem (or Biology) before, here are the numbers for net ATP production for a molecule of d-glucose, from start to finish:

Glucose --> 2 Pyruvate = 2 ATP + 2 NADH
2 Pyruvate --> 2 Acetyl-CoA = 2 NADH
2 Acetyl-Coa through TCA --> 2 x (1 GTP, 1 FADH2, 3 NADH) = 2 GTP + 2 FADH2 + 6 NADH

That gives us: 4 ATP equivalents + 10 NADH + 2 FADH2

Oxidative P-lation:

10 NADH * 2.5 ATP/NADH = 25 ATP
2 FADH2 * 1.5 ATP/FADH2 = 3 ATP
+ 4 ATP equivalents from before = 25 + 3 + 4 = 32 net ATP
--------


Now, my question: sometimes, we take into account the ATP cost of moving the 2 NADH's from the cytosol (those produced in glycolysis) into the mito. matrix. That happens at a cost of, according to my records, 1 ATP per NADH...

Does that mean that our actual net production is only 30 ATP?
Additionally, are there cells that do not have to 'pay' to get NADH into the mito. matrix?
Are there cells that are 'better' at ATP production then others, that will get us more net ATP?

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it depends on the shuttle-system used to transport electrons from the cytosol into the ETC.

If the malate-aspartate shuttle-system is used (liver, heart, kidneys), then cytosolic NADH pumps 10 H+ into the inter membrane space and cytosolic NADH is equivalent to matrix NADH -> 32 ATP / 1 glucose.

If the glycerol-3-phosphate shuttle-system is used (brain, skeletal muscle), then cytosolic NADH only pumps 6 H+ into the inter membrane space and cytosolic NADH is equivalent to matrix FADH2 -> 30 ATP / 1 glucose.
 
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