Passage excerpt:
Topic: Sickle cell anemia is caused by two copies of the allele for abnormal hemoglobin, HbS
A. Yes, because the mutation causing sickle-cell anemia in the HbS gene eliminated the MstII site.
B. Yes, because the probe specific for the 1.1-kb fragment successfully hybridized with the 1.3-kb fragment.
C. No, because the 1.3-kb fragment from the HbS gene would not normally hybridize with the probe specific for the 1.1-kb fragment of the HbA gene.
D. No, because mutations other than the one responsible for HbS production could have eliminated the MstII site.
Can someone explain why each is wrong/right? Thank you much in advance!
Topic: Sickle cell anemia is caused by two copies of the allele for abnormal hemoglobin, HbS
Q: A DNA sample is digested by MstII, yielding a 1.3-kb fragment that is then successfully hybridized with a probe specific for the 1.1-kb fragment of the HbA gene. Does this prove that the DNA sample contains the HbS gene?...In addition, when the gene for the β chain of HbA (normal hemoglobin) is digested by the restriction enzyme MstII, a 1.1-kb fragment is generated. When the gene for the β chain of HbS, which lacks a MstII site, is digested by MstII, a 1.3-kb fragment is generated
A. Yes, because the mutation causing sickle-cell anemia in the HbS gene eliminated the MstII site.
B. Yes, because the probe specific for the 1.1-kb fragment successfully hybridized with the 1.3-kb fragment.
C. No, because the 1.3-kb fragment from the HbS gene would not normally hybridize with the probe specific for the 1.1-kb fragment of the HbA gene.
D. No, because mutations other than the one responsible for HbS production could have eliminated the MstII site.
Can someone explain why each is wrong/right? Thank you much in advance!