1. Visit this thread to beta-test StudySchedule.org. StudySchedule is a free nonprofit site that builds dynamic MCAT study schedules unique for your needs and timeline.
Check out the new Application Assistant, where you can calculate your LizzyM score, see how you rank compared to other applicants, and see a list of schools where similar students were accepted.

Quick Q about electroblotting!

Discussion in 'Pre-Medical - MD' started by Dannyk1992, Sep 30, 2014.

  1. SDN is made possible through member donations, sponsorships, and our volunteers. Learn about SDN's nonprofit mission.
Thread Status:
Not open for further replies.
  1. Does anyone know why a heavier protein would transfer during electroblotting but a smaller protein wouldn't?
    Shouldn't smaller proteins electroblot more easily?

    thanks!
     
  2. SDN Members don't see this ad. About the ads.
  3. Goro

    Goro Probationary Status 5+ Year Member

    Depends upon how small the smaller protein was....maybe it ran off the gel?

    The things >100kDa tend to be the ones that have more trouble blotting.

     
  4. hoihaie

    hoihaie Banned Banned

    1,821
    1,780
    Mar 6, 2014
    what kind of gel ae you using? gradient?
     
  5. 911 Turbo

    911 Turbo MS-1 Gold Donor 5+ Year Member

    How do you know your protein didn't transfer? How much of the protein are you expecting in the sample?
     
  6. Ismet

    Ismet PGY-fun! SDN Administrator 5+ Year Member

    9,531
    8,887
    May 15, 2011
    Physician
    SDN is not for homework help. Closing thread.
     

Thread Status:
Not open for further replies.

Share This Page