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Quick Q about electroblotting!

Discussion in 'Pre-Medical Allopathic [ MD ]' started by Dannyk1992, 09.30.14.

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  1. Dannyk1992

    Dannyk1992 2+ Year Member

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    Does anyone know why a heavier protein would transfer during electroblotting but a smaller protein wouldn't?
    Shouldn't smaller proteins electroblot more easily?

    thanks!
     
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  3. Goro

    Goro 5+ Year Member

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    Depends upon how small the smaller protein was....maybe it ran off the gel?

    The things >100kDa tend to be the ones that have more trouble blotting.

     
  4. hoihaie

    hoihaie Banned Banned

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    what kind of gel ae you using? gradient?
     
  5. 911 Turbo

    911 Turbo MS-1 Gold Donor 5+ Year Member

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    How do you know your protein didn't transfer? How much of the protein are you expecting in the sample?
     
  6. Ismet

    Ismet PGY-fun SDN Administrator 5+ Year Member

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    SDN is not for homework help. Closing thread.
     

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