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Goro

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Depends upon how small the smaller protein was....maybe it ran off the gel?

The things >100kDa tend to be the ones that have more trouble blotting.

Does anyone know why a heavier protein would transfer during electroblotting but a smaller protein wouldn't?
Shouldn't smaller proteins electroblot more easily?

thanks!
 

hoihaie

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what kind of gel ae you using? gradient?
 

911 Turbo

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How do you know your protein didn't transfer? How much of the protein are you expecting in the sample?
 

Ismet

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SDN is not for homework help. Closing thread.
 
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