1. Visit this thread to beta-test StudySchedule.org. StudySchedule is a free nonprofit site that builds dynamic MCAT study schedules unique for your needs and timeline.
Hey, Guest, do you know how much will it cost you to apply to medical school? Check out SDN's Medical School Application Cost Calculator and plan your budget.

Quick Q about electroblotting!

Discussion in 'Pre-Medical - MD' started by Dannyk1992, Sep 30, 2014.

  1. SDN is made possible through member donations, sponsorships, and our volunteers. Learn about SDN's nonprofit mission.
Thread Status:
Not open for further replies.
  1. Does anyone know why a heavier protein would transfer during electroblotting but a smaller protein wouldn't?
    Shouldn't smaller proteins electroblot more easily?

  2. SDN Members don't see this ad. About the ads.
  3. Goro

    Goro 7+ Year Member

    Depends upon how small the smaller protein was....maybe it ran off the gel?

    The things >100kDa tend to be the ones that have more trouble blotting.

  4. hoihaie

    hoihaie Banned Banned

    Mar 6, 2014
    what kind of gel ae you using? gradient?
  5. 911 Turbo

    911 Turbo med student Gold Donor 5+ Year Member

    Jul 24, 2010
    hSDN Member
    hSDN Alumni
    How do you know your protein didn't transfer? How much of the protein are you expecting in the sample?
  6. Ismet

    Ismet PGY-fun! SDN Administrator 5+ Year Member

    May 15, 2011
    SDN is not for homework help. Closing thread.

Thread Status:
Not open for further replies.

Share This Page