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Quick Q about electroblotting!

Discussion in 'Pre-Medical - MD' started by Dannyk1992, Sep 30, 2014.

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  1. Does anyone know why a heavier protein would transfer during electroblotting but a smaller protein wouldn't?
    Shouldn't smaller proteins electroblot more easily?

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  3. Goro

    Goro Faculty 7+ Year Member

    Jun 10, 2010
    Somewhere west of St. Louis
    Depends upon how small the smaller protein was....maybe it ran off the gel?

    The things >100kDa tend to be the ones that have more trouble blotting.

  4. hoihaie

    hoihaie Banned Banned

    Mar 6, 2014
    what kind of gel ae you using? gradient?
  5. 911 Turbo

    911 Turbo med student Classifieds Approved 7+ Year Member

    Jul 24, 2010
    hSDN Member
    hSDN Alumni
    How do you know your protein didn't transfer? How much of the protein are you expecting in the sample?
  6. Ismet

    Ismet PGY-fun! Administrator Physician 5+ Year Member

    May 15, 2011
    SDN is not for homework help. Closing thread.

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