I am doing research as part of my senior thesis in a laboratory where things are suddenly turning very sucky. What do I do? The story is that I attempted a Cesium Chloride DNA isolation(for the first time)which didn't work and the PI is so angry that he told the post-doc I work with that its either I get it right or I leave his lab. I am not worried about getting it right, because I've identified the source of error that the lab tech (the guru of the technique)missed. He basically told me I could stop the proceedure at any point and go to class if I had to. Current literature as I find out does not echo what I was told.Anyway, the big question is should I go talk to the PI or just get it right and shutup? I have completed the bulk of my research and I am really focusing on the write -up. That's not to say I'm not interested in sequencing the targeting constructs that my research has been based on. I have been really upset since I heard this story and really don't know what to do? I know the lab has financial problems and cannot afford to use the QIAGEN kits anymore. The lab tech is apparently the only person in the lab who does this technique--even my post-doc has no clue about it!Should I just suck it up and not say a word? I want to play my cards right and not screw up my relation with the lab.