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- Jul 9, 2015
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Hi all, came across a practice question that gave me 3 carb. acids with overlapping boiling points and asked me to pick which was bets way to separate them all.
A. extraction separation using aqueous KOH.
B. HPLC with polar solvent.
C. fractional distillation.
D. SDS electrophoresis.
They say the answer is D, because the acids are only meaningfully different in their molecular weights/size, which SDS-PAGE can exploit. I know this is used to separate AA chains and nucleic acids, but can you use it to separate individual acids from one another? would the answer need to say the pores are sufficiently small?
A. extraction separation using aqueous KOH.
B. HPLC with polar solvent.
C. fractional distillation.
D. SDS electrophoresis.
They say the answer is D, because the acids are only meaningfully different in their molecular weights/size, which SDS-PAGE can exploit. I know this is used to separate AA chains and nucleic acids, but can you use it to separate individual acids from one another? would the answer need to say the pores are sufficiently small?