What's the difference between DNA polymerase I and III

Discussion in 'DAT Discussions' started by ROCOCO8, Jan 12, 2012.

  1. SDN is made possible through member donations, sponsorships, and our volunteers. Learn about SDN's nonprofit mission.
  1. ROCOCO8

    ROCOCO8 5+ Year Member

    97
    0
    Aug 12, 2011
    I'm a little confused about this..
    What's the difference bw I and III? .I always thought E. coli/bacteria only have DNA poly III...?
     
  2. SDN Members don't see this ad. About the ads.
  3. Blue87Fiero

    Blue87Fiero 2+ Year Member

    239
    5
    Sep 21, 2010
    I don't know which ones E.Coli has for sure but I thought I, II, and III all came from E.Coli. I actually thought it went up to five. I think DNA III was the biggest DNA Pol I was unique because it could "proof read" in both directions 5' to 3' and 3' to 5'. I am not sure if I am right on this I might check tomorrow if I remember. I just finished genetics and I should know this.
     
  4. ROCOCO8

    ROCOCO8 5+ Year Member

    97
    0
    Aug 12, 2011
    Oh well that makes more sense... But which type of polym do humans use?
     
  5. Ferferi

    Ferferi 2+ Year Member

    100
    15
    Aug 29, 2011
    Los Angeles, CA
    I wasn't sure about the answer so I googled it and this is what I got

    DNA Polymerase III - makes the chain in a 5 to 3 direction and it proofreads with a 3 to 5 exonuclease, so it goes both ways doing something different
    DNA Polymerase I - breaks down the RNA primer with a 5 to 3 exonuclease.
     
  6. Dotoday

    Dotoday 7+ Year Member

    1,560
    87
    May 27, 2007
    The Molecular bio nomenclature states that DNA Polymerase I, II, and III is used in procaryotes like E-Coli. Eucaryotes use DNA Polymerase alpha, beta, delta, epsilon.

    DNA Polymerase III is responsible for leading and the lagging strand synthesis in bacteria. DNA Polymerase I replaces RNA primer with the DNA strand.

    Sent from my MB855 using Tapatalk
     

Share This Page