Theoretically, they should not ask.
Realistically, they might. And perhaps innocently. Let's say I see lots of neuro rotations on your transcript, and a neuro research project. Then you apply to my IM program. When I interview you, I might ask "So, megatron, it looks like you used to be interested in neuro and now you're interested in IM. What's up with that?". The discussion that follows may be tricky for you. You can lie all you want -- if you ultimately match in Neuro I'll know it (as I see where everyone on my rank list ultimately matches). But there's nothing I can "do" about it, and honestly at that point I won't care. Every year a few on my list end up matching in Derm, or Ophthal. And every year I look to see if I could have avoided wasting my time interviewing them by looking carefully at their apps.