Free floating adenine vs ATP

[Curse]

---

Members don't see this ad.

I understand that ATP has deoxyribose sugar, 3 phosphates and an adenine nucleotide, but how is that different from an adenine nucleotide that is freefloating in structure? because im having difficulty with confirming whether ATP is directly incorporated into a structure rather than its energetic uses. Before being put into a DNA molecule, it also has a deoxyribose, 3 phosphates and an adenine and pyrophosphate is removed rather than 1 phosphate when ATP is hydrolyzed.
Thanks in advanced

 

[tn4596]

from what i remmeber from my biochem class..
Adenine is a base. Idk whether it is free floating or stored somewhere in the cell (we never talk about that) When it is connected to a ribose ring it is called a nucleoside. When a nucleoside is connected to 1-3 phosphates, it become a nucleotide, hence ATP.
ATP, as well as GTP, UTP CTP, can be used in DNA replication. the process i think use a enzyme called theoxine reductase....rly long mechanism and take off one of the -OH group in the ribose ring, making it deoxylribose (this is done because the 3'OH can cause DNA to degrade as seen in RNA...you should google RNA self cleavage if you are interested in the mechanism)
and then the 5' end of the elongating DNA strand just attack the dATP (with deoxylribose) (or dGTP etc.) adding a AMP and leaving pyrophosphate..(which is 2 phospates). This process is long and require lots of enzyme (see DNA Replication)
When ATP is hydrolyzed...an entirely diffrent mechanism..ATP => ADP + P...pretty simple because ATP contain lots of energy...
ATP as well as other nucleotide can also be used in a bunch of other chemical reaction that I cannot simply remmeber on top of my head...so I guess that the answers yo ur looking for...

 

[Majik]

I understand that ATP has deoxyribose sugar, 3 phosphates and an adenine nucleotide, but how is that different from an adenine nucleotide that is freefloating in structure? because im having difficulty with confirming whether ATP is directly incorporated into a structure rather than its energetic uses. Before being put into a DNA molecule, it also has a deoxyribose, 3 phosphates and an adenine and pyrophosphate is removed rather than 1 phosphate when ATP is hydrolyzed.
Thanks in advanced

ATP (Adenosine 5' Triphophate) consists of 3 Phosphates, 1 Ribose (not deoxyribose) Sugar, and an Adenine Base. It's true that ATP is also incorporated during transcription when DNA is transcribed to RNA, just as GTP, UTP, and CTP are as well. However, aside from forming RNA, ATP is used widely throughout the body as a form of energy, catalyzing several unfavorable reactions (positive deltaG). It can do this by hydrolyzing 2 bound phophates (a pyrophosphate) forming AMP -- which is much more energetically favorable (highly negative deltaG), or by releasing 1 phosphate group and forming ADP.

 

[collegestud2013]

ATP = adenosine 5'-triphosphate. This is the ATP used for harnessing energy (by adding to water to hydrolyze the phosphoanhydride bond to ADP and Pi) and incorporated into mRNA during transcription.

dATP = 2'-deoxy-adenosine-5'triphosphate. This dATP is incorporated into DNA during replication.

ddATP = 2',3'-dideoxy-adenosine-5'-triphosphate. This is the ddATP used during the Sanger chain termination DNA sequencing method.

So the differences are whether he 2' carbon on the pentose sugar is an -H or -OH.