Help! Reading frames in plasmid!

[greenteapudding]

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It's probably a stupid question...please help! I'm working on converting a cloning vector to a gateway destination vector for LR reaction!

I'm trying to insert a invitrogen LR reaction attR site into a plasmid by restriction enzyme (blunt ended), but i dunno which reading frame i should be using for the fragment. There are three cassettes of the attR fragment to choose. But I have trouble understanding how to identify the reading frame of the plasmid, and how to keep the reading frame by choosing the right cassette...

Here's the kit info and manual:
https://catalog.invitrogen.com/inde...//www.invitrogen.com/content.cfm?pageid=10287

Any help would be appreciated...Thanks!

 

[Ultra7]

They can help, and I think you're better off listening to them in case you have other issues or it doesn't work.
Good luck!