isoenzymes vs alternative splicing

[super linrondo]

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i was review aamc 11 and got confused on isoenzymes. i know that isoenzymes are coded by different genes, my question is why can't they be the result of alternative splicing? is there anyway to know if two different proteins are results of alternative splicing or different DNA sequence?

thanks a lot everyone!!!

 

[dontbeanegaton]

It's just a definition issue. Isozymes have different genes by definition. The term is interchangeable with allele.
Alternate splicing of enzymes doesn't make alleles/isozymes. It just makes long and short forms. There's no actual variation in the sequence within exons, just the exclusion/inclusion of them.

 

[Temperature101]

It would be strange if they have alternative splicing on the MCAT...People who do not take Genetics might not know anything about alternative splicing.

 

[kevin2400]

I've definitely seen alternative splicing pop up a few times during my studies. Though not very likely to be tested, I do think the general idea of pre-mRNA processing is good to know and would probably go hand in hand, since the 5' cap and poly-A tail is listed on the AAMC content outline, but beyond that I don't think you are expected to know more, and any more information would be given in the passage.

 

[deleted103644]

It's just a definition issue. Isozymes have different genes by definition. The term is interchangeable with allele.
Alternate splicing of enzymes doesn't make alleles/isozymes. It just makes long and short forms. There's no actual variation in the sequence within exons, just the exclusion/inclusion of them.

Not necessarily. You can have alternative exons in the middle and end up with multiple transcripts of approximately the same length.

There's a lot more complexity to splicing than an occasional skipped exon.