Kaplan is confusing me on what should be a simple bio concept

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Nik2012

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What parts of cellular respiration are considered substrate-level phosphorylation and what parts are oxidative phos? I am confused.

Is the ETC oxidative while glycolysis, pyruvate decarb and krebs are all substrate-level? I have bigger things to worry about than this but it's driving me crazy b/c I keep getting confused.

Please help me.

Oh, one more thing while I'm posting. What is a really good strategy for tackling the reading section of the DAT? I've decided it will be impossible for me to read all three passages intently and have enough time to answer all the ?'s. right?
 
I believe "substrate level Phos. only" = the direct phosphorolation of ADP -> ATP vs the coupled reactions of NADH and FADH2 we have in e- transport chain which oxidises NADH and FADH2 to create a proton gradient, that gradient drives the ADP ----> ATP phosphorlation (oxidative phos.).

So the two substrate only reactions where ADP is directly phosphorolated to give a NET of ATP are:
Glycolysis (cytosol): which yields a net 2 ATP/1 Glucose molecule (note that this substrate level phos. happens several times here but only yields a "net" of 2 ATP)
Krebs Cycle (Mit. Matrix): net 2 ATP (1 per cycle actually in the form of GTP which transfers its Pi to ADP --> ATP)

So a net of only 4 ATP from substrate level phosphorolation.

All the other ATPs are generated through oxidative phosphorolation in the e- transport chain by taking the reduced NADH and FADH2 molecules from Glycolysis (2 NADH), PDC (2 NADH), Krebs (6 NADH, 2FADH2), and oxidizing them pumping protons OUT of the Mit. matrix (increasing pH in the matrix) into the inner mit. membrane. This gradiant (with mit. membrane proteins) then drives the phosphorolation of ATP production. This is where the bulk of ATP comes from.

Ok after a few edits I think I got this right, I just covered it yesterday so it was fresh on my mind. Feel free to add any info.

Also I dont know what to do for reading either, I am the slowest test taker there is. I spent 8 hours on an O-Chem test one time when the class avg. was 2.5. The few undergrad classes that had timed tests were always the toughest, instead of an A-, B+ student I was dropped to a B- student. My g-chem class for example, I was #1 in my class of 72 all semester until the final which was 25 questions and a 2 hour limit. I missed the last 6 questions b/c I didn't finish :-( dropping my A- to a B (not even a B+).

This is why I have put off the dat for so long, I should have taken it and applied last year or earlier. I am studying 12-16 hrs/day right now but my battle in EVERY section by far is time. My brain just doesn't function well under time constraints, I don't know if it is my ADD or what but, I can't even reason well and answer stuff that I know sometimes, so I sympathise with you as I am in the same boat, or worse. My test date is Oct. 1 (24 days away) so I am just trying to do as many practice tests as possible etc.
 
wow, thanks so much for the great summary! Looks perfect!

I was just looking thru Kaplan's diagnostic and on #4's answer (of the bio portion) it says "oxidative phosphorylation is the process by which ATP is synthesized via the Krebs cycle and ETC." Ahhh! I think it must be a typo because in Kaplan's lesson book it breaks down cell respiration and only mentions the ETC as being part of ox. phos. (pg. 70)

thanks again for the help!!
 
I take mine on Sept. 19 so I have 11 measely days left! I'm doing the same thing you are, just taking as many practice tests as I can and hope for the best!

Thanks again for your help. Wonderful!!
 
What parts of cellular respiration are considered substrate-level phosphorylation and what parts are oxidative phos? I am confused.

Is the ETC oxidative while glycolysis, pyruvate decarb and krebs are all substrate-level? I have bigger things to worry about than this but it's driving me crazy b/c I keep getting confused.

Please help me.

Oh, one more thing while I'm posting. What is a really good strategy for tackling the reading section of the DAT? I've decided it will be impossible for me to read all three passages intently and have enough time to answer all the ?'s. right?


RC strategy is to skim while writing key words down.. especially dates and numbers for EACH paragraph that is numbered. that way when u read the question, it will have it. its' best to skim cuz of those darn tone questions which u might get. good luck! practice is everything.
 
I've been doing 10 min reading/10 min answering questions. It's been working for all my practice tests. I don't know how it's going to be on the real thing😕
 
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