number aa to DNA nucleotide

[vvvv]

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question in destroyer ask: how many DNA nucleotides need to make a protein contained 210 amino acids.

a. 210
b. 70
c. 630
d. 1000
e. 1500
answer is 630 (210 x 3)

However, when I took genetics this kind of question always used and the answer should not be 630. because according to my professor, he said when 210 aa is translated, the mRNA contain some nucleotides that are not translated (exon and intron for example) so does the DNA.

On the DNA, some intron and exon will be transcript to make aa but on exon is translated. so the answer always do like this. 210 x 3 = 630 nucleotides on mRNA and 630 x 2 = 1260 becasue DNA is double stranded and it contain intron so answer should be higher than 1260 which is 1500

what you guys think?

 

[osimsDDS]

question in destroyer ask: how many DNA nucleotides need to make a protein contained 210 amino acids.

a. 210
b. 70
c. 630
d. 1000
e. 1500
answer is 630 (210 x 3)

However, when I took genetics this kind of question always used and the answer should not be 630. because according to my professor, he said when 210 aa is translated, the mRNA contain some nucleotides that are not translated (exon and intron for example) so does the DNA.

On the DNA, some intron and exon will be transcript to make aa but on exon is translated. so the answer always do like this. 210 x 3 = 630 nucleotides on mRNA and 630 x 2 = 1260 becasue DNA is double stranded and it contain intron so answer should be higher than 1260 which is 1500

what you guys think?

yea you are right, but you dont know if its a bacterial DNA molecule or an animal DNA molecule...the difference is in RNA processing...where bacterial mRNA doesnt have introns only exons so splicing of introns would not occur and the DNA molecules are directly proportional to the aa of the polypeptide protein...

I am not saying your wrong but I would just assume they are not talking about splicing and all that stuff cuz it would be impossible to find out how many nucleotides were spliced out as introns and which were kept as exons if you know what i mean...the sequence of introns to exons is random there is no way of knowing from the problem...

 

[bumpski20]

I agree with you that the answer shouldnt be 630. if they are talking about complete cDNA the answer should be 630 + 3 (for the stop codon). im not really sure about the 1500. im pretty sure you can't tell how many nucleotides are in the DNA because like you said, there are introns that will be spliced out and you can't tell how many base pairs long the introns are. also, you don't know how many base pairs long the UTR's are. any1 else agree?

 

[bumpski20]

osims you beat me to it!! but question...does bacterial DNA contain any UTRs?

 

[osimsDDS]

osims you beat me to it!! but question...does bacterial DNA contain any UTRs?

hmm i dunno about that but they do contain a 5' cap and poly A tail right...or not??? I forgot that part...I do know tho that they do transcription and translation at the exact same time simultaneously as the RNA is being transcribed so would that mean that they dont have a t' cap and poly A tail??? thanks

 

[harrygt]

question in destroyer ask: how many DNA nucleotides need to make a protein contained 210 amino acids.

a. 210
b. 70
c. 630
d. 1000
e. 1500
answer is 630 (210 x 3)

However, when I took genetics this kind of question always used and the answer should not be 630. because according to my professor, he said when 210 aa is translated, the mRNA contain some nucleotides that are not translated (exon and intron for example) so does the DNA.

On the DNA, some intron and exon will be transcript to make aa but on exon is translated. so the answer always do like this. 210 x 3 = 630 nucleotides on mRNA and 630 x 2 = 1260 becasue DNA is double stranded and it contain intron so answer should be higher than 1260 which is 1500

what you guys think?

I would say, forget about exons and interons because we don't know.
210 + 1 [stop codon] = 211 codons.
211 codons x 3 = 633 ribonucleotides

DNA is double stranded, so we wil have 1266 nucleotides on DNA.
However I don't see the asnwer in any of the choices. I'm sure the answere can't be around 600. it's double that!

 

[bumpski20]

yeah i know transcription and translation are coupled for bacteria. I think it may depend on the bacteria but im pretty sure most ha*e little to no processing (processing being the cap, splicing and tail). the reason I think so is because the purpose of the coupled transcription and translation is to protect the RNA. RNA degrades much easier then DNA and protein and since the cap and the tail protect eukaryotic RNA from the degrading enzymes in the cytoplasm, im pretty sure transcription and translation are coupled in bacterial cells because they don't ha*e this protection.

 

[WayneSU]

question in destroyer ask: how many DNA nucleotides need to make a protein contained 210 amino acids.

a. 210
b. 70
c. 630
d. 1000
e. 1500
answer is 630 (210 x 3)

However, when I took genetics this kind of question always used and the answer should not be 630. because according to my professor, he said when 210 aa is translated, the mRNA contain some nucleotides that are not translated (exon and intron for example) so does the DNA.

On the DNA, some intron and exon will be transcript to make aa but on exon is translated. so the answer always do like this. 210 x 3 = 630 nucleotides on mRNA and 630 x 2 = 1260 becasue DNA is double stranded and it contain intron so answer should be higher than 1260 which is 1500

what you guys think?

Another reason why this answer is wrong, i think, is because on top of the 630 nucleotides needed to translate the 210 aa's, you need at least three more nucleotides for a stop codon.

 

[osimsDDS]

question:

You know how in eukaryotic cells the 5' cap of mRNA in rna processing is GTP and the phosphates are the groups that give the ribosomes the energy to bind...this obviously is on the 5' end...but doesnt translation go 3' to 5' of the mRNA because the tRNA goes 5' to 3' or is it just that the mRNA is translated 5' to 3' ??? haha i just got totally confused but if someone answers i will be set just need to know this part thanks

 

[vvvv]

bacteria have a 5'UTR similar with 5'cap in eukary...it called shine dalgarno sequence

 

[Glycogen]

I think,we have to assume that the interon are already out of the picture and we are dealing with whatever came out of the nucleuse to be translate,so in this case no need to doubt about number which is 630.Am I right?

 

[bumpski20]

question:

You know how in eukaryotic cells the 5' cap of mRNA in rna processing is GTP and the phosphates are the groups that give the ribosomes the energy to bind...this obviously is on the 5' end...but doesnt translation go 3' to 5' of the mRNA because the tRNA goes 5' to 3' or is it just that the mRNA is translated 5' to 3' ??? haha i just got totally confused but if someone answers i will be set just need to know this part thanks

haha polarities suck. the tRNA reads starting from the 5' end of the mRNA to the 3' end. I just remember which way the strands are read from: synthesis> DNA is read 3-5
transcription> DNA is read 3-5
translation> mRNA is read 5-3.

 

[bumpski20]

I think,we have to assume that the interon are already out of the picture and we are dealing with whatever came out of the nucleuse to be translate,so in this case no need to doubt about number which is 630.Am I right?

but if its out of the nucleus its not DNA, its some type of RNA. and they are still forgetting about the stop codon. I really dont think they can expect us to assume theres no such thing as introns and UTRs. im sticking with my original claim, we cant solve this.

 

[DCRedskinsRule]

In my opinion, I think everyone is thinking WAY too hard about this question. Look at the answer choices. First of all, 630 is the only feasible one, it's not like they had 630 and 633 or w/e.

Second of all, I think everyone is making the question too hard. The question may be worded ambiguously but I think they're simply asking you to recognize that for each AA, you need 3 nucleotides b/c a codon is 3 nt's long.

Rmbr for all you out there studying the DAT....the purpose is to get the points and the questions right. If the answer is obviously correct like the above question, don't waste your time with in depth understanding of "oh is it bacterial or animal." It's not in the question so you don't need to worry about it. Get your points and save your time! Best of luck! 👍

 

[Mamona]

I agree with osimsDDS

 

[bumpski20]

In my opinion, I think everyone is thinking WAY too hard about this question. Look at the answer choices. First of all, 630 is the only feasible one, it's not like they had 630 and 633 or w/e.

Second of all, I think everyone is making the question too hard. The question may be worded ambiguously but I think they're simply asking you to recognize that for each AA, you need 3 nucleotides b/c a codon is 3 nt's long.

Rmbr for all you out there studying the DAT....the purpose is to get the points and the questions right. If the answer is obviously correct like the above question, don't waste your time with in depth understanding of "oh is it bacterial or animal." It's not in the question so you don't need to worry about it. Get your points and save your time! Best of luck! 👍

I dont no...i mean going into the test you really should have a solid understanding of genetics. If you are given a protein length you should understand the the original DNA should be MUCH longer (usually more then half the original DNA is not translated into the protein).

 

[vvvv]

In my opinion, I think everyone is thinking WAY too hard about this question. Look at the answer choices. First of all, 630 is the only feasible one, it's not like they had 630 and 633 or w/e.

Second of all, I think everyone is making the question too hard. The question may be worded ambiguously but I think they're simply asking you to recognize that for each AA, you need 3 nucleotides b/c a codon is 3 nt's long.

Rmbr for all you out there studying the DAT....the purpose is to get the points and the questions right. If the answer is obviously correct like the above question, don't waste your time with in depth understanding of "oh is it bacterial or animal." It's not in the question so you don't need to worry about it. Get your points and save your time! Best of luck! 👍

So that mean we need to lower our understanding in order to answer the question right?

 

[Simiam]

In my opinion, I think everyone is thinking WAY too hard about this question. Look at the answer choices. First of all, 630 is the only feasible one, it's not like they had 630 and 633 or w/e.

Second of all, I think everyone is making the question too hard. The question may be worded ambiguously but I think they're simply asking you to recognize that for each AA, you need 3 nucleotides b/c a codon is 3 nt's long.

Rmbr for all you out there studying the DAT....the purpose is to get the points and the questions right. If the answer is obviously correct like the above question, don't waste your time with in depth understanding of "oh is it bacterial or animal." It's not in the question so you don't need to worry about it. Get your points and save your time! Best of luck! 👍

I agree with DC... If the answer choices that they give you are set up like that, they are obviously wanting the answer to be 630. 633 would be a more correct answer due to the stop codon NT, but even you could take it more in depth. Of the answer choices possible you should be logical and realize they are asking a simple question and the answer is 630.

The logic for responding with 1500 on the DAT in my mind would be going beyond the context of the qestion. From what I have seen and studied they are trying to be as straight forward as possible and testing your basic knowledge of the information. Just my opinion though.

 

[Orgodox]

I would say, forget about exons and interons because we don't know.
210 + 1 [stop codon] = 211 codons.
211 codons x 3 = 633 ribonucleotides

DNA is double stranded, so we wil have 1266 nucleotides on DNA.
However I don't see the asnwer in any of the choices. I'm sure the answere can't be around 600. it's double that!

Even if you wanted to include the stop codon your math includes it twice.

Im with DC you guys are way over-thinking this problem. Its not meant to be tricky at all.

 

[bumpski20]

So that mean we need to lower our understanding in order to answer the question right?

i guess so because im 100% positive that it will have more then 630. these kind of questions are so lame!!

 

[DCRedskinsRule]

I dont no...i mean going into the test you really should have a solid understanding of genetics. If you are given a protein length you should understand the the original DNA should be MUCH longer (usually more then half the original DNA is not translated into the protein).

In answer to this and vvvv's comment....no. I'm not saying do not understand the concept. I'm saying don't waste your time during the test going through every concept under the umbrella of the topic. Focus on the one, isolated, discrete question and answer it directly. If you do that, for the question posted above, the answer is 630, and you can get that answer within 20 seconds, and move on. Whether you agree with me or not honestly doesn't really matter... I'm just trying to help those who are currently studying and feel as though time is an issue for the science section.

 

[Simiam]

question in destroyer ask: how many DNA nucleotides need to make a protein contained 210 amino acids.

a. 210
b. 70
c. 630
d. 1000
e. 1500
answer is 630 (210 x 3)

Another way to look at it is to understand that the DNA sequences used to make a protein may be 20000 nucleotides long, but if the protein contains only 210 amino acids then only 630 nucletotides were needed to make the protein. The rest of the DNA sequence was not needed and thus was spliced out.

 

[bumpski20]

Another way to look at it is to understand that the DNA sequences used to make a protein may be 20000 nucleotides long, but if the protein contains only 210 amino acids then only 630 nucletotides were needed to make the protein. The rest of the DNA sequence was not needed and thus was spliced out.

just because they are spliced out doesnt mean they are not needed!!

 

[bumpski20]

In answer to this and vvvv's comment....no. I'm not saying do not understand the concept. I'm saying don't waste your time during the test going through every concept under the umbrella of the topic. Focus on the one, isolated, discrete question and answer it directly. If you do that, for the question posted above, the answer is 630, and you can get that answer within 20 seconds, and move on. Whether you agree with me or not honestly doesn't really matter... I'm just trying to help those who are currently studying and feel as though time is an issue for the science section.

I agree with you on what your saying but I don't agree with answer...if that makes sense. This one question particularly frustrates me because its clearly wrong but on test day if i got a question like this I would most likely chose 630 (hopefully). haha i know i just condradicted myself a bunch of times. basically im trying to say your right but the answer is still wrong

 

[Simiam]

just because they are spliced out doesnt mean they are not needed!!

No, but it means that they are not used in making that particular protein...

 

[DCRedskinsRule]

I agree with you on what your saying but I don't agree with answer...if that makes sense. This one question particularly frustrates me because its clearly wrong but on test day if i got a question like this I would most likely chose 630 (hopefully). haha i know i just condradicted myself a bunch of times. basically im trying to say your right but the answer is still wrong

That I completely understand 😀

 

[AndyK]

Even in bacterial genomes the genes have longish regions at the beginning and the end of the gene so that there is somewhere for the ribosome to attach and so that the sequence can be terminated, respectively.

But if I was presented with that question I would definately KISS it (keep it simple stupid 🙂)