Argh.. I thought I had this done. But may be not.
I thought that when:
pI > pH -- the amino acid is protonated and receives a partial or full positive charge --> attracted to cathode (-)
pI < pH -- the amino acid is deprotonated and receives a partial or full negative charge --> attracted to anode (+)
but I don't get why basic amino acids have a higher pI. Wouldn't they be a zwiterion when they are in a lower pH???
or is it because basic amino acids would be so willing to receive a H+ that they would do so even when H+ is more scarce (higher pH)??
Sorry if the question is convoluted.. got really confused 😕
I thought that when:
pI > pH -- the amino acid is protonated and receives a partial or full positive charge --> attracted to cathode (-)
pI < pH -- the amino acid is deprotonated and receives a partial or full negative charge --> attracted to anode (+)
but I don't get why basic amino acids have a higher pI. Wouldn't they be a zwiterion when they are in a lower pH???
or is it because basic amino acids would be so willing to receive a H+ that they would do so even when H+ is more scarce (higher pH)??
Sorry if the question is convoluted.. got really confused 😕