PRP expiration?

[bedrock]

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Is there a consensus on how long the PRP can sit before an injection until the PRP is compromised?

1hr?

2hr?

 

[Agast]

Do you mean in terms of utility or in terms of infection risk? I would use the same guidelines as blood transfusion products. Try to administer within 30 minutes.

https://www.sciencedirect.com/science/article/abs/pii/S0887796311000939#:~:text=The%2030%2Dminute%20rule%20states,removal%20from%20controlled%20temperature%20storage.

 

[Ducttape]

why would there be any delay of using it?

didnt you just draw their blood and spin it down and have them sitting in your waiting room or exam room?

 

[BobBarker]

I had a rep tell me about getting PRP in his scalp for hair loss. He said they drew his blood, made the PRP, injected him, then took the extra and stored it in a fridge for next time. Scary.

 

[Agast]

why would there be any delay of using it?

didnt you just draw their blood and spin it down and have them sitting in your waiting room or exam room?

There are other patients that need to be seen while the PRP is processing though

 

[BobBarker]

My workflow for PRP is to draw blood and start processing and then immediately take the patient back and get access. It takes long enough to get the patient to the procedure room, prep, get access that the PRP is ready to use just as I am ready to inject it.

 

[bedrock]

Thanks. I do all of the above.

I’m just wondering how much efficacy is lost after a certain timeframe and if there is any data on it. Calling @drusso

 

[Ducttape]

hmm....

maybe longer than we all expect:

Platelet-rich plasma for tissue regeneration can be stored at room temperature for at least five days - PubMed

Although platelet integrity and PDGF-BB fell over time, the intermittently agitated non-oxygen permeable container appeared to retain better platelet integrity and function, and PDGF-BB release, than other storage conditions, with potential for clinical use for 5-8 days.

pubmed.ncbi.nlm.nih.gov

Conclusion: Although platelet integrity and PDGF-BB fell over time, the intermittently agitated non-oxygen permeable container appeared to retain better platelet integrity and function, and PDGF-BB release, than other storage conditions, with potential for clinical use for 5-8 days.

equine:

Short term storage stability at room temperature of two different platelet-rich plasma preparations from equine donors and potential impact on growth factor concentrations - PubMed

L-PRP ad modum E-PET and P-PRP ad modum ACP® are applicable over at least a six hours time span at room temperature without loss of growth factor content. Based on the results of this study factors influencing the resulting growth factor concentrations still remain questionable. Additional...

pubmed.ncbi.nlm.nih.gov

6 hours

Conclusions: L-PRP ad modum E-PET and P-PRP ad modum ACP® are applicable over at least a six hours time span at room temperature without loss of growth factor content.

Formulation and Storage of Platelet-Rich Plasma Homemade Product

The platelet-rich plasma (PRP) is an autologous biotherapy based on platelet-healing properties. Here, we developed a simple and reproducible PRP purification protocol based on two successive centrifugations. We evaluated different centrifugation speeds ...

www.ncbi.nlm.nih.gov

About 130 and 250 g successive speed centrifugations further concentrated platelets while preserving their bioactivity during 6 h (after that, platelet functions were significantly altered). In these conditions, we obtained a highly concentrated pure PRP product (with a low leukocyte count) suitable to study platelet properties. To avoid the loss of efficacy, we recommend injecting PRP under 3 h after preparation.