rflp

[nycfella]

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Can someone explain this to me in layman's then in scientific lingo.

rest. frag. length. polymorphism's

 

[DrJD]

Can someone explain this to me in layman's then in scientific lingo.

rest. frag. length. polymorphism's

Here is an example of rflp used for forensic science...

You take the suspects DNA and you chop it up with enzymes that are specific to certain short palindromic pieces of DNA and you get a bunch of chunks of different lengths. Then you use the enzymes to chop up the DNA of blood from the crime scene and it too is then in little chunks.

Next you put both of these chopped up DNA bits on a gel and run them. Gel Electrophoresis separates based on size of the fragments. So based on the specific DNA it will be in different sized chunks. When you run the gel, the fragments will separate and give you a "Fingerprint" of sorts. You can then compare the different wells and see if they came from the same DNA.

Hope that helps was in "layman" enough!