Using Pubmed to find restriction sites of a DNA sequence

[chondrocyte]

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hey all,

is there a way to use Pubmed/Blast to show whether there are are any restriction sites (for Eco R1) on a DNA sequence?

need to find out whether EcoR1 will cut at any point on my DNA sequence - if so, I can't use this restriction enzyme

thanks 🙂

 

[SticknRudder]

I use this:

http://www.restrictionmapper.org/

 

[cathyg]

hey all,

is there a way to use Pubmed/Blast to show whether there are are any restriction sites (for Eco R1) on a DNA sequence?

need to find out whether EcoR1 will cut at any point on my DNA sequence - if so, I can't use this restriction enzyme

thanks 🙂

I'm pretty sure BLAST will not do this for you. I've used this site successfully: http://rna.lundberg.gu.se/cutter2/index.html

 

[xanthines]

I use this:

http://searchlauncher.bcm.tmc.edu/seq-util/seq-util.html

-X

I use this:

http://www.restrictionmapper.org/

 

[JDAD]

www.neb.com then pick webcutter - works well

 

[leverp2000]

Yeah, neb cutter works very well.

www.neb.com then pick webcutter - works well

 

[Mitro]

Yeah, neb cutter works very well.

NEBcutter or bust

 

[dr.z]

You can get DNA Strider. It works well too.