I thought it didnt but from the section bank, in one problem they seem to think that it does. Does anyone know for sure?
I thought it didnt but from the section bank, in one problem they seem to think that it does. Does anyone know for sure?
The homotrimers will break up under normal (non-reducing) SDS-PAGE because the three identical subunits must be linked with either one or a combination of hydrogen bonds, hydrophobic surface interactions, and/or salt bridges. The SDS detergent will disrupt these interactions and result in three separate unassociated peptide chains.