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663697
Tissue staining with specific antibodies, known as immunohistochemical analysis, did NOT show increased levels of pStat3 (phosphorylated Stat3 transcription factor). What explanation is LEAST likely to account for this finding?
A. pStat3 is denatured during the tissue preparation process
B. Low grade primary tumors were stained instead of malignant tumors
C. Anti-pStat3 antibody also recognizes pStat5 (correct)
D. A secondary antibody-enzyme conjugate was not used in the experiment
I got this correct but for the wrong reason. I thought that the antibody would be specific to pStat3 so it wouldn't bind pStat5, but the answer says binding to pStat5 is possible, except it would produce a greater signal, contrary to what's stated in the question stem. I always thought the antibody was specific to a given antigen.. is this incorrect?
A. pStat3 is denatured during the tissue preparation process
B. Low grade primary tumors were stained instead of malignant tumors
C. Anti-pStat3 antibody also recognizes pStat5 (correct)
D. A secondary antibody-enzyme conjugate was not used in the experiment
I got this correct but for the wrong reason. I thought that the antibody would be specific to pStat3 so it wouldn't bind pStat5, but the answer says binding to pStat5 is possible, except it would produce a greater signal, contrary to what's stated in the question stem. I always thought the antibody was specific to a given antigen.. is this incorrect?
