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Hi everyone, I was wondering if anyone could help me with this question: DNA samples from an HbAA and an HbSS individual are digested with restriction endonucleases, separated by gel electrophoresis and exposed to a hybridization probe. Southern blotting shows 2 bands for the HbSS sample, but only one for the HbAA sample. Which is the most likely explanation?
The answer is: HbSS sample contains a restriction site while the HbAA doesn't.
My question is: why isn't it that the HbSS sample contains 2 restriction sites while HbAA contains 1?
Thanks!
The answer is: HbSS sample contains a restriction site while the HbAA doesn't.
My question is: why isn't it that the HbSS sample contains 2 restriction sites while HbAA contains 1?
Thanks!