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I think it's certainly possible to get in without post-grad experience, but being that it's so competitive, those who have that extra experience will probably have a better chance getting in (if all other qualifications are similar). I think it doesn't hurt to apply, and if you don't get in, take 2 years to get some experience and apply again.
I worked for 3 years at the National Insitute of Health doing research, and 2 years at Columbia University doing research (I took so much time mostly because I didn't know what I wanted to do yet). This is my first time applying, and I haven't heard anything yet, but I am hoping that my experience will give me an edge over other people....