In one of the section bank questions, the correct answer was to determine the least effective method whereby one would determine the localization of a protein (pRB) within a cell.
The Correct answer (and therefore the least effective method) was to label a probe that would hybridize to the transcript of said protein.
I'm aware of the fact that it means it will pretty much be sitting in the cytosol where the ribosome is located, but is the correct that it's because once translation occurs, the fluorescent probe would come off? AAMC didn't specifically mention this but its the only way I can reason this out because if it stays on the translated protein, wouldn't it just be like tagging the protein itself? (Which was one of the answer choices; which was deemed as an effective method).
The Correct answer (and therefore the least effective method) was to label a probe that would hybridize to the transcript of said protein.
I'm aware of the fact that it means it will pretty much be sitting in the cytosol where the ribosome is located, but is the correct that it's because once translation occurs, the fluorescent probe would come off? AAMC didn't specifically mention this but its the only way I can reason this out because if it stays on the translated protein, wouldn't it just be like tagging the protein itself? (Which was one of the answer choices; which was deemed as an effective method).