Nadh + h+

[yjj8817]

When nad+ becomes Nadh + h+ in glycolysis, where does the extra h+ come from?

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[swollcat]

You're treading into metabolic biochemistry but if you must know the minutiae, PGAL aka a triose sugar is dehydrogenated and contributes it's H to the reduction of NAD+ -> NADH

 

[azor ahai]

When NAD+ gets reduced, it takes a hydride (H-) which is where the two electrons comes from, while it gets rid of a proton (H+).

 

[erythrocyte666]

+1 to above two, except NAD+ doesn't deprotonate; the proton comes from the sugar