questions from official guide

[hopeful2019]

Hi,
Passage 3 of the biological and biochemical section talks about the protozoan Plasmodium falciparum. I have a question based on that passage. How did they find the substrate binding domain in pfSET10? I did not get their explantions where they say fragment lacking the PHD domain but containing the SET domain does not bind unmodified histone H3, how did they figure that based on the diagram? I mean I saw the - sign for SET/delta PHD, but dont knw how it gets interpreted?
Thanks!

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[bee17]

The passage says that PfSET10 modifies histone H3. Therefore, histone H3 is the substrate. If the PfSET10 fragment with SET and PHD binds H3 but the fragment with just SET and no PHD does not, then PHD must be the substrate binding domain.