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After I solubilize and purify insulin receptor from human placentae using sepharose-insulin column chromatography, I'd want to confirm the bioactivity and affinity of the solubilized, purified receptor. I could do a binding affinity experiment using 125I labelled insulin in the presence or absence of unlabelled insulin.
My question is, now that the insulin receptor is solubilized, how do I separate the free vs bound labelled insulin before measuring the radioactivity??
My question is, now that the insulin receptor is solubilized, how do I separate the free vs bound labelled insulin before measuring the radioactivity??