Help with this question? The answer is D, but the explanation makes no sense to me. I thought western blot was used to analyze proteins, so what does nucleotide hybridization have anything to do with this?
I see how they would use antibody for this, but why is it called "nucleotide" hybridization? There's no nucleotide involved, only proteins that we are analyzing and antibody(also made of protein) that we are hybridizing it with.The proteins are identified using hybridization probes - in a Western blot these are antibodies that bind to the specific proteins, this is the nucleotide hybridization step.
Ok, so the only reason this reason is correct is because it mentions "hybridization"?I'm not completely sure why they are saying nucleotide hybridization, when antibody probes are used in a Western Blot not nucleotide probes. Nucleotide probes are northern/southern blots so either they are implying that there is a relationship between that reaction and the hybridization step in a western blot, or it is a mistake and they meant northern/southern blot. D is the only answer even close though.