aa sequence

[cmb81]

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where can i find the amino acid seq./ protein seq. for sp1 (aa 622-788)?

 

[Doctor&Geek]

http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=protein&val=38372901

 

[cmb81]

thanks, but i dont think that is the right one...the sequence only goes up to 785 aa....i need 622-788. i used pGEX 2TK vector to get GST-Sp1 fusion protein complex and now i need the aa sequence to continue to the next step.

 

[Doctor&Geek]

The one I linked to is H. sapiens RefSeq curated by NCBI.

Likely some isoform, polymorphism, or difference in sequencing is behind the differences in mRNA and protein sequences deposted at NCBI. I would wager you don't need aa 785-788 if all you want to do is purify proteins to make antibodies or do protein interaction or kinase studies.

 

[mendel121]

The one I linked to is H. sapiens RefSeq curated by NCBI.

Likely some isoform, polymorphism, or difference in sequencing is behind the differences in mRNA and protein sequences deposted at NCBI. I would wager you don't need aa 785-788 if all you want to do is purify proteins to make antibodies or do protein interaction or kinase studies.

I'm really not a fan of NCBI's site, try goldenpath at http://genome.ucsc.edu, then type in the gene name to the search box at the genomes link. This will bring up all of the isoforms, including the ones that are often hidden (at least to me) in NCBI. I would look at more than one of the sequences to make sure that it actually starts with a methionine, all to often the protein sequence is simply based on the cDNA, and if the cDNA doesn't start with ATG then neither will the protein (often, not always). If the protein sequence doesn't start with M, then the numbering will be off. Also you should use the accession number for the sequence that you got the aa numbers you need from. Each accession number may have slightly different sequences, and so different numbering.