Affinity Chromatography

[MedPR]

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What would be the best way to separate a solute, call it B, from a mixture of A, B, and C? A is the smallest, B is middle, and C is the largest.

The best thing I can think of is to do two separate runs of affinity chromatography. The first one with beads small enough for A to adhere to, but not B and C. Now you have a solution of only B and C. Then do a second round of affinity chromatography with a column that would let C pass, but not B. Then you just have to elute the column to have B isolated. Then remove whatever you eluted it with and have pure solute B.

Is there a simpler way?

 

[chiddler]

knowing only their size, this doesn't give you many options.

i think what you wrote is the only thing that is possible with nothing else.

 

[mcloaf]

You've confused affinity chromatography with size-exclusion chromatography. For size-exclusion, which works exactly how it sounds, your method would be fine. Affinity chromatography is more specific and is usually used for isolating proteins. Examples would be using his-tags, avidin-biotin binding, or antigenic interactions--basically, something super specific for the thing you're trying to isolate.

 

[SaintJude]

Yeah, does affinity chromatography have anything to do with size even? I think it's more related to the biological composition, b/c you coat the the column of bread with receptors, antibodies or biological targets associated with your compound of interest.

 

[mcloaf]

Yeah, does affinity chromatography have anything to do with size even? I think it's more related to the biological composition, b/c you coat the the column of bread with receptors, antibodies or biological targets associated with your compound of interest.

Precisely.