Any insteresting microogranism living in mouth that I can study use SEM?

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Smooth Operater

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Hello guys! My biology prof is going to select one student from her class to perform any research the student wants on a Scanning Electron Microscope. She will teach the student to use the microscope first, then the student has to perform a research on his/her chosen topic and write a paper about it later. I am interested to perform a research related to oral biology (perhapes studying a type of micro-granims in mouth) using a SEM. But, I am only a 2nd yr univeristy undergrad, so I don't know any "HOT" and "INTERESTING" oral biology subjects atm that I can look into. The project should focus on form and function relationship of a specieman. I hope you guys can provides some suggestions on what I can research on using a Scanning Electron Microscope. It will difinitely help me tremedously on writing my proposal! 👍 Thank you very much!
 
Smooth Operater said:
Hello guys! My biology prof is going to select one student from her class to perform any research the student wants on a Scanning Electron Microscope. She will teach the student to use the microscope first, then the student has to perform a research on his/her chosen topic and write a paper about it later. I am interested to perform a research related to oral biology (perhapes studying a type of micro-granims in mouth) using a SEM. But, I am only a 2nd yr univeristy undergrad, so I don't know any "HOT" and "INTERESTING" oral biology subjects atm that I can look into. The project should focus on form and function relationship of a specieman. I hope you guys can provides some suggestions on what I can research on using a Scanning Electron Microscope. It will difinitely help me tremedously on writing my proposal! 👍 Thank you very much!
You might consider investigating the structure of salivary glands. I believe this is a hot topic currently.
 
Hello guys! I have to perfrom a research on a mcrioorganism using Scanning Electron Microscope. Any insteresting microogranism living in mouth that I can study use SEM?
 
Smooth Operater said:
Hello guys! I have to perfrom a research on a mcrioorganism using Scanning Electron Microscope. Any insteresting microogranism living in mouth that I can study use SEM?

Candida is a good one to study. We have lots of people studying that here at Iowa. Look up K Vargas on pubmed and you'll get lots of SEM images of candida under different types of stress.
 
bitecys said:
Candida is a good one to study. We have lots of people studying that here at Iowa. Look up K Vargas on pubmed and you'll get lots of SEM images of candida under different types of stress.

Hey btecys! I couldn't located the article you refer to by looking up K Vargas. If I don't find something to look at, I probably will end up probing my own saliva to see what stuffs are there without any specific purpose. Maybe someone can can ask a question regarding saliva that i can try to find out using SEM?
 
Smooth,

I am an experienced SEM user and have recently spent 8 weeks at the NIH/NIDCR using SEM to study macrophage-T-Cell interactions. How is this related to dentistry? We pre-treated several of these monocytes/macrophages with secretory leukocyte protease inhibitor (SLPI) - which is found in the oral cavity and functions in wound healing.

In the past, I also spent time at Univ. of Miami working in a SEM lab studying foot fungi (T. rubrum) with my brother who is a podiatrist. The work from the fungus project has been published in the Journal of the American Podiatric Medical Association (July/August 2004).

As a student studying SEM, there are countless numbers of ideas you can work on related to oral biology. Some critters are easier to manipulate under the scope than others, however, it all depends on what you have access to. For example, if you can get your hand on teeth, you can look at dentinal tubules, enamel pores, pulpal tissue, etc. If you can get fungus or bacteria, they are great projects available but you run into more preparation concerns than just using teeth.

How much time do you have for your class? Time constraints, access to scope & preparation materials, and most importantly, access to specimens dictates the project.

SEM + Dentistry = great match
There is a good amount of dental science being done with SEM around the country, a majority related to dental materials research (i.e. how filling materials fit to teeth, etc.)

If you need any help in preparation protocols for bugs, fungi, teeth, do ask.

Best of luck,
-Mike
 
Smooth Operater said:
Hello guys! My biology prof is going to select one student from her class to perform any research the student wants on a Scanning Electron Microscope. She will teach the student to use the microscope first, then the student has to perform a research on his/her chosen topic and write a paper about it later. I am interested to perform a research related to oral biology (perhapes studying a type of micro-granims in mouth) using a SEM. But, I am only a 2nd yr univeristy undergrad, so I don't know any "HOT" and "INTERESTING" oral biology subjects atm that I can look into. The project should focus on form and function relationship of a specieman. I hope you guys can provides some suggestions on what I can research on using a Scanning Electron Microscope. It will difinitely help me tremedously on writing my proposal! 👍 Thank you very much!

As a former microbiology grad, I remember that one of my professors (http://www.mcgill.ca/microimm/department/professors/chan/ ) who taught at both the faculty of dentistry and microbiology, studied spirochetes quite a bit. It wasn't until I went through dental school that I realized that spirochetes were quite implicated in periodontal disease .Spirochetes can be studied with SEM. A notorious spirochete (at least periodontally) : Treponema denticola
 
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