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- Jul 22, 2011
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So I've been working at a particular lab for two years (including summers) and I really wonder why I haven't quit yet. When I started working at my lab, the lab people would always talk about how my PI was weird, but I never really understood why they said that. Now I do.
So I've been working on an experiment that a postdoc conceptualized regarding a cell line and treatment with a ubiquitinating drug. The postdoc just wanted to see if using one drug had an effect on cell growth in a twelve well plate, but the PI thought it was a very useless experiment and wanted us to just get proteins from the cells in a six inch plate. So I was stuck in the middle trying to do both experiments for both people. The thing is I tried a drug concentration on the cells that was based off the twelve well plate experiment that we did. The cells didn't really die from the drug concentration that I used. So I told my PI this and he just blew up at me in his office and told me that I was wasting my time with this stupid experiment. He told me to get out of the office and told me to think of a better way of finishing the experiment. Ugh, I just wanted to pack my bags up and leave. I was simply telling him what I observed, and especially since this wasn't an experiment that I conceptualized, why should I have taken the blame for it? On top of that, the PI came into the lab afterwards and was talking happily to the postdoc I work with as if nothing happened.
My PI is generally a good person, but he has weird moments and always misunderstands what I say to him. 😡 Does anyone else have experiences like this?
So I've been working on an experiment that a postdoc conceptualized regarding a cell line and treatment with a ubiquitinating drug. The postdoc just wanted to see if using one drug had an effect on cell growth in a twelve well plate, but the PI thought it was a very useless experiment and wanted us to just get proteins from the cells in a six inch plate. So I was stuck in the middle trying to do both experiments for both people. The thing is I tried a drug concentration on the cells that was based off the twelve well plate experiment that we did. The cells didn't really die from the drug concentration that I used. So I told my PI this and he just blew up at me in his office and told me that I was wasting my time with this stupid experiment. He told me to get out of the office and told me to think of a better way of finishing the experiment. Ugh, I just wanted to pack my bags up and leave. I was simply telling him what I observed, and especially since this wasn't an experiment that I conceptualized, why should I have taken the blame for it? On top of that, the PI came into the lab afterwards and was talking happily to the postdoc I work with as if nothing happened.
My PI is generally a good person, but he has weird moments and always misunderstands what I say to him. 😡 Does anyone else have experiences like this?