Bio Destroyer #280 Identify Pic Q

[tawaqul]

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It has a picture of a structure taken from a mature white blood cell by an e- microscope.

The answer is lysosome, but why can't it be a nucleus?

 

[tranv117]

not sure but I want to say that the nucleus would have RER around it...

 

[Albuterol]

well my reasoning would be because the picture is stained so darkly and its freaking huge it has to be lysosome. A WBC contains the organelle lysosome. Since a WBC has phagocytic activity its going to engulf foreign particles via phagocytosis and form a phagosome. The phagosome will fuse with the lysosome and then the lysosome will degrade the material inside the phagosome. The lysosome is continously working due to the phagocytic activity of the wbc and will be enlarged.

 

[tranv117]

can you really conclude that that organelle is large? There really isnt a proper scale for that in the slide. But I do agree with your reasoning with the stain and it being a WBC.

 

[tawaqul]

why would a lysosome be stained darkly and not a nucleus?

 

[Albuterol]

ok forget it being large b/c the picture is zoomed in by 1000x lol i was going off memory of what it looked like. but yea i defintiely saw that it stained darkly and part of a mature WBC and was able to conclude it was a lysosome.

these questions kinda bug me though, i had a friend take her DAT last yr and get a slide question and she said it was pretty hard. Hopefully we won't get something like that.

 

[tranv117]

why would a lysosome be stained darkly and not a nucleus?

well i was thinking that not much can be stained in the nucleus other than chromatin which you should see a lot of faint string like flourescence under a flourescence microscope. But even then, the nucleus is so small that the entire thing with look stained anyways. so not sure about that.

 

[Albuterol]

agree with above.