Chymotrypsin

[wannabedocta]

So where exactly does chymotrypsin cleave? and what about trypsin? It's not explained in my mcat review books, but I remember encountering a question testing this knowledge. It is at the carboxyl side of AA's with phenyl rings? another source says leucine also?

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[iA-MD2013]

chymotrypsin recognizes amino acids with aromatic rings: phenylalanine, tryptophan, and tyrosine. I think it weakly recognizes leucine...but i doubt that would ever matter (it didnt even matter in my biochem class...let alone the mcat).
trypsin recognizes positively charged amino acids: lysine and arginine (it has a negative charge on its active site).
Both cleave at the carboxyl end of the specified amino acids.
Im not sure if this would matter for the mcat though...?

 

[wannabedocta]

Thanks a lot!!!