DAT Bio Question

[Djdents]

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Which of the following is the major intracellular buffer?

a. TRIS
b. Acetate
c. Phosphate
d. Bicarbonate
e. Calcium Carbonate

I chose c but the answers say that the correct answer is d. Can anyone explain?

 

[Soleus715]

Which of the following is the major intracellular buffer?

a. TRIS
b. Acetate
c. Phosphate
d. Bicarbonate
e. Calcium Carbonate

I chose c but the answers say that the correct answer is d. Can anyone explain?

Bicarbonate is the major (EDIT: EXTRACELLULAR not intracellular) buffer by definition. Note how it is amphoteric and is able to act as acid or base.

This helps regulate the pH of blood.

http://en.wikipedia.org/wiki/Bicarbonate

I see that you have a 22 on the DAT. That is a great score. Are you thinking of retaking?

 

[ICUrunning]

Hey guys
Actually you are both wrong, what practice test is this from DJdents?

From DAT Destroyer
#325
This phosphate buffer system operates in the internal fluids of all cells. H2PO4- and HPO42- act as the acid and base respectively. Phosphate is an abundant anion in cells and will act as the major intracellular buffer. The pH of the extracellular fluid is maintained by the bicarbonate buffer ie. HCO3-/H2CO3 system

hope this helps and yeah your scores are great, dont retake imo!

 

[Soleus715]

Good call, you're completely right. I even typed out intracellular and didn't realize what I was doing.

Controlling the pH of blood would mean that it is an extracellular and not intracellular buffer.

Thanks 👍

BTW, from memory I feel like I had this question in one of the bio bootcamp tests.

 

[Faux]

Hey guys
Actually you are both wrong, what practice test is this from DJdents?

From DAT Destroyer
#325
This phosphate buffer system operates in the internal fluids of all cells. H2PO4- and HPO42- act as the acid and base respectively. Phosphate is an abundant anion in cells and will act as the major intracellular buffer. The pH of the extracellular fluid is maintained by the bicarbonate buffer ie. HCO3-/H2CO3 system

hope this helps and yeah your scores are great, dont retake imo!

so qvault is wrong?

 

[ICUrunning]

Not sure where the original question is from but all you need to know is
1. Phosphate buffer - intracellular
2. bicarbonate buffer - extracellular

I dont remember seeing this question in qvault but is definitely on DATBootcamp

 

[Dotoday]

It is absolutely Bicarbonate. That is the only buffer you have in your cells. Phosphate, is not a cell buffer. It can be a buffer in a test tube, not in ghe cell. Phosphate in the cell is used for Phosphorylation of protein, sugars or ADP to ATP synthesis.

 

[Djdents]

I am not retaking haha. So the trick in the question is that it says phosphate rather than phosphate buffer system?

 

[Djdents]

Hey guys
Actually you are both wrong, what practice test is this from DJdents?

From DAT Destroyer
#325
This phosphate buffer system operates in the internal fluids of all cells. H2PO4- and HPO42- act as the acid and base respectively. Phosphate is an abundant anion in cells and will act as the major intracellular buffer. The pH of the extracellular fluid is maintained by the bicarbonate buffer ie. HCO3-/H2CO3 system

hope this helps and yeah your scores are great, dont retake imo!

It's from some Canadian practice test