Dilution Question

[golfinpanda]

---

Members don't see this ad.

So I'm currently growing 5 bacterial cell cultures. I start at the dilution of 10^-1 by adding 300 microliters of a media and 33 microliters of the bacterium. So when we do these dilutions fewer cells are developed. Is it because we are starting with fewer cells or is it because we are using less spent media?