In EK bio pg 32, it says "When heated or immersed in high concentration salt solution or high pH solution, the hydrogen bonds connecting the two strands in a double stranded DNA molecules are disrupted, and the strands separate; the DNA molecule is called the denatured or melted."
Is it true that high salt solution will denature the dsDNA? Or is this one of the common errors in EK books? I learned in my class that high salt solution shields negative charge repulsion between phosphate backbones, thus making dsDNA more stable.
Help please.
Is it true that high salt solution will denature the dsDNA? Or is this one of the common errors in EK books? I learned in my class that high salt solution shields negative charge repulsion between phosphate backbones, thus making dsDNA more stable.
Help please.
