Step I DNA Replication FC

[RP29]

Disclaimer: These questions might seem elementary, sorry in advance.

In FC:

*DNA Pol α: acts as a RNA primase, elongates the primer w/ 20 DNA nucleotides, after which DNA Pol δ takes over

*DNA Pol δ: analogous to DNA Pol III, also w/ 3’→5’ exonuclease proofreading

^I was under the impression that DNA pol delta was used for the lagging strand whereas DNA pol epsilon is used for the leading strand. Is there a reason that DNA pol epsilon isn't mentioned?

*Telomerase: enzyme that has an intrinsic RNA primer and synthesizes DNA upstream of the 5’ end → no shortening of chromosomes during replication

^I was under the impression that telomerase adds to the 3' end of the parental strand. Can someone comment on this?


Thank You.

 

[Brain Bucket]

Disclaimer: These questions might seem elementary, sorry in advance.

In FC:

*DNA Pol α: acts as a RNA primase, elongates the primer w/ 20 DNA nucleotides, after which DNA Pol δ takes over

*DNA Pol δ: analogous to DNA Pol III, also w/ 3’→5’ exonuclease proofreading

^I was under the impression that DNA pol delta was used for the lagging strand whereas DNA pol epsilon is used for the leading strand. Is there a reason that DNA pol epsilon isn't mentioned?

*Telomerase: enzyme that has an intrinsic RNA primer and synthesizes DNA upstream of the 5’ end → no shortening of chromosomes during replication

^I was under the impression that telomerase adds to the 3' end of the parental strand. Can someone comment on this?


Thank You.

I know this isn't helpful, but when we were studying this last year, we did pepper our Molecular Bio Prof with Qs...her response was that a lot of these don't have a straight answer and that neither she nor the NBME would ask about nitpicky details on the euk polys. You gotta know your prok stuff like rho factor though.