"DNA strand with most nucleotides"

[virtualmaster999]

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Hey everyone!

I kinda got thrown up on this. Seems like it could be 2 answers:

From a fragment of DNA, which of the following molecules contains the least amount of nucleotides?

• A. The primary RNA transcript from the original DNA
• B. A single strand of the original DNA
• C. A single strand of the original DNA after a nonsense mutation
• D. A single strand of cDNA from the original DNA
• E. A single strand of the original DNA after a missense mutation

So I know cDNA is intron free, but how would you know it would have less nucleotides than a strand from a nonsense mutation, since the stop codon could be very early on?

Is there a way to distinguish this?

Thanks in advance!

 

[whitepapertree]

Hey everyone!

I kinda got thrown up on this. Seems like it could be 2 answers:

From a fragment of DNA, which of the following molecules contains the least amount of nucleotides?

• A. The primary RNA transcript from the original DNA
• B. A single strand of the original DNA
• C. A single strand of the original DNA after a nonsense mutation
• D. A single strand of cDNA from the original DNA
• E. A single strand of the original DNA after a missense mutation

So I know cDNA is intron free, but how would you know it would have less nucleotides than a strand from a nonsense mutation, since the stop codon could be very early on?

Is there a way to distinguish this?

Thanks in advance!

You have a fragment of DNA. All DNA are the same size in this case.

Just because the DNA has a stop codon doesn't mean it has less nucleotides (it's still got three making up that code but that's beside the point). The resultant protein would have less AA, but that's not the question.

However, with no introns then there is less nucleotides in the fragment of DNA.

 

[virtualmaster999]

You have a fragment of DNA. All DNA are the same size in this case.

Just because the DNA has a stop codon doesn't mean it has less nucleotides (it's still got three making up that code but that's beside the point). The resultant protein would have less AA, but that's not the question.

However, with no introns then there is less nucleotides in the fragment of DNA.

Thanks for that explanation, helped a lot!