Does SDS PAGE separate the two units of a homodimer?

[LIC2015]

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Native PAGE vs SDS Page for a homodimer would look identical, but would there be two separated protein in the one SDS PAGE band?

 

[theonlytycrane]

If it's a homo-dimer, the two identical units would appear at a band 1/2 the weight as shown in the Native Page once separated.

 

[LIC2015]

If it's a homo-dimer, the two identical units would appear at a band 1/2 the weight as shown in the Native Page once separated.

You're saying SDS breaks the homodimer into its two identical units, right?

 

[theonlytycrane]

Yeah, one way or another. If the homo-dimer is connected by disulfide bond(s), reducing SDS will be needed. Otherwise just denaturing SDS will break the homo-dimer into two identical units.