This question seems simple but im not sure what im missing here. The passage states that the enzyme is active as a homotetramer. It is subjected to SDS page which "confirmed the purity and showed an active band at 35 kda"
It asks for the molecular weight of the compound.
The ans is 140kDa. I thought the answer would be 35 kda because the SDS page only shows one band, so i assumed thats the combined weight of the homotetramer. Why is my reasoning flawed?
It asks for the molecular weight of the compound.
The ans is 140kDa. I thought the answer would be 35 kda because the SDS page only shows one band, so i assumed thats the combined weight of the homotetramer. Why is my reasoning flawed?