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EK Biology: In-Class Exam Lecture 2, Question 32 (from passage II)
In R looping the displaced DNA strand contains the complementary nucleotide sequence for:
A) the entire DNA strand shown in figure 2 [correct]
B) the entire RNA strand shown in figure 2
C) the DNA introns only
D) the RNA introns only
I picked C. My reasoning: the R looping technique involves using a fully processed mRNA strand (contains only exons) and hybridizing with a double stranded DNA counterpart to form hybrids between DNA and RNA strands. "In this process, the RNA strand displaces one DNA strand and binds to the other DNA strand along complementary sequences."
So, the DNA strand that hybridizes to the RNA is exonic DNA sequences (binding to the exonic/processed mRNA). Thus, the displaced DNA strand - the loops - must represent DNA introns.
I thought the "loops" are the displaced DNA strands - if so then based on question 31, shouldn't the answer to 32 be C as well?
Thanks!!!
In R looping the displaced DNA strand contains the complementary nucleotide sequence for:
A) the entire DNA strand shown in figure 2 [correct]
B) the entire RNA strand shown in figure 2
C) the DNA introns only
D) the RNA introns only
I picked C. My reasoning: the R looping technique involves using a fully processed mRNA strand (contains only exons) and hybridizing with a double stranded DNA counterpart to form hybrids between DNA and RNA strands. "In this process, the RNA strand displaces one DNA strand and binds to the other DNA strand along complementary sequences."
So, the DNA strand that hybridizes to the RNA is exonic DNA sequences (binding to the exonic/processed mRNA). Thus, the displaced DNA strand - the loops - must represent DNA introns.
I thought the "loops" are the displaced DNA strands - if so then based on question 31, shouldn't the answer to 32 be C as well?
Thanks!!!