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If you have TBR please look at Bio Section 2, Passage 14 concerning Sickle Cell Anemia and HbF.
Please look at question #91. TBR says the answer to diagnose Sickle Cell Anemia is by RFLPs on red blood cell DNA and gel electrophoresis of hemoglobin samples from red blood cells.
HOW can RFLP tests be done on red blood cell DNA when RBC do not have DNA? I think TBR made a mistake here. I think the answer should be II only. Can anyone confirm?
Also, on question #92, TBR says HbF does not bind 2,3-BPG, but in my biochem textbook it says that HbF does bind 2,3-BPG to a lesser degree than adult HB. Is this too another area of discrepancy?
Please confirm.
Please look at question #91. TBR says the answer to diagnose Sickle Cell Anemia is by RFLPs on red blood cell DNA and gel electrophoresis of hemoglobin samples from red blood cells.
HOW can RFLP tests be done on red blood cell DNA when RBC do not have DNA? I think TBR made a mistake here. I think the answer should be II only. Can anyone confirm?
Also, on question #92, TBR says HbF does not bind 2,3-BPG, but in my biochem textbook it says that HbF does bind 2,3-BPG to a lesser degree than adult HB. Is this too another area of discrepancy?
Please confirm.